Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma.
Antecedentes: la toma de muestras de sangre es un factor importante que puede influir los resultados finales de algunas pruebas de laboratorio. Las determinaciones de acilcarnitinas en sangre son utilizadas corrientemente para el diagnóstico y seguimiento de pacientes que sufren enfermedades metabólicas hereditarias. Objetivo: el presente estudio analizó la posible influencia del tipo de anticoagulante utilizado en la toma de la muestra de sangre, sobre el perfil de acilcarnitinas obtenido. Metodología: muestras de sangre y plasma fueron obtenidas utilizando heparina, potasio-EDTA, fluoruro de sodio-oxalato de potasio, y citrato de sodio y sin anticoagulante. Resultados: no fue encontrada diferencia estadísticamente significativa entre los... Ver más
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Revista Biosalud - 2010
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Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. Universidad de Caldas Artículo de revista Núm. 2 , Año 2010 : Julio - Diciembre 2 9 Biosalud espectrometría de masas en tándem acilcarnitinas carnitina Osorio, José Henry Antecedentes: la toma de muestras de sangre es un factor importante que puede influir los resultados finales de algunas pruebas de laboratorio. Las determinaciones de acilcarnitinas en sangre son utilizadas corrientemente para el diagnóstico y seguimiento de pacientes que sufren enfermedades metabólicas hereditarias. Objetivo: el presente estudio analizó la posible influencia del tipo de anticoagulante utilizado en la toma de la muestra de sangre, sobre el perfil de acilcarnitinas obtenido. Metodología: muestras de sangre y plasma fueron obtenidas utilizando heparina, potasio-EDTA, fluoruro de sodio-oxalato de potasio, y citrato de sodio y sin anticoagulante. Resultados: no fue encontrada diferencia estadísticamente significativa entre los anticoagulantes utilizados. Conclusión: el tipo de anticoagulante no tiene efecto sobre la determinación de acilcarnitinas en sangre o plasma. Para este tipo de análisis, puede ser utilizado cualquier anticoagulante de los antes mencionados. Abreviaturas: IEE-MS/MS, inyección de electrospray espectrometría de masas en tándem; EIM, errores innatos del metabolismo. Osorio JH, Pourfarzam M. Plasma free and total carnitine measured in children by tandem mass spectrometry. Braz J Med Biol Res 2002;35(11):1265-71. Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010;603:9-25. Kodo N, Millington DS, Norwood DL, Roe CR. Quantitative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1990 Jan 31;186(3):383-90. Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010;44(2):15-20. Holub M, Tuschl K, Ratschmann R, Strnadová KA , Mühl A , Heinze G, et al. Influence of hematocrit and localisation of punch in dried blood spots on levels of amino acids and acylcarnitines measured by tandem mass spectrometry. Clin Chim Acta 2006; 373:27–31. Sewell AC. Methods of carnitine analysis. In: Seim H, Löster H, editors. Carnitine-Pathobiochemical Basics and Clinical Applications. Bochum: Ponte Press 1996: 73-84. Osorio JH, Pourfarzam M. Determination of normal acylcarnitine levels in a healthy pediatric population as a diagnostic tool in inherited errors of mitochondrial fatty acid beta-oxidation. An Pediatr (Barc) 2007;67(6):548-52. info:eu-repo/semantics/publishedVersion Schoderbeck M, Auer B, Legenstein E, Genger H, et al. Pregnancy-related changes of carnitine and acylcarnitine concentrations of plasma and erythrocytes. J Perinat Med 1995;23:477-85. Mancinelli A, Iannoni E, Calvani M. Effect of temperature on the stability of long-chain acylcarnitines in human blood prior to plasma separation. Clin Chim Acta 2007; 375:169-170. info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Nagaraja D, Mamatha SN, De T, Christopher R. Screening for inborn errors of metabolism using automated electrospray tandem mass spectrometry: study in high-risk Indian population. Clin Biochem 2010;43(6):581-8. http://purl.org/coar/version/c_970fb48d4fbd8a85 info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 Text Stevens RD, Hillman SL, Worthy S, Sanders D, Millington DS. Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 2000;46(5):727-9. Publication Journal article Revista Biosalud - 2010 https://creativecommons.org/licenses/by-nc-sa/4.0/ Inglés Efecto del tipo de anticoagulante utilizado en la toma de muestras, sobre la determinación del perfil de acilcarnitinas en sangre y plasma. https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498 Background: the collection process for blood sampling is a very important factor that can influence the final results of some laboratory tests. The acylcarnitine determinations in blood are usually used for diagnosis and follow up of patients suffering certain inherited metabolic illnesses. Objective: this study analyzed the possible influence of the type of anticoagulant used in blood sampling on the acylcarnitine profile. Methodology: blood and plasma samples were collected using anticoagulants such as: lithium heparin, potassium EDTA, sodium fluoride-potassium oxalate, and sodium citrate. Samples were also collected without the use of anticoagulants. Acylcarnitine levels were assessed for each sample via tandem mass spectrometry. Results: no significant differences were found among the anticoagulant used. Conclusion: the type of anticoagulant has no effect on the acylcarnitine determination on blood or plasma. For this type of analysis any of the above mentioned anticoagulants can be used. Abbreviations: ESI-MS/MS, electrospray injection tandem mass spectrometry; IEM, innate errors of metabolism. carnitine application/pdf acylcarnitines tandem mass spectrometry 13 2022-03-17 2022-03-17T00:37:02Z 2022-03-17T00:37:02Z https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/5498/4965 9 1657-9550 2462-960X https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498 |
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UNIVERSIDAD DE CALDAS |
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https://nuevo.metarevistas.org/UNIVERSIDADDECALDAS/logo.png |
| country_str |
Colombia |
| collection |
Biosalud |
| title |
Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. |
| spellingShingle |
Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. Osorio, José Henry espectrometría de masas en tándem acilcarnitinas carnitina carnitine acylcarnitines tandem mass spectrometry |
| title_short |
Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. |
| title_full |
Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. |
| title_fullStr |
Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. |
| title_full_unstemmed |
Effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. |
| title_sort |
effect of anticoagulant type during the blood simple collection process on the determination of the acylcarnitine profile in blood and plasma. |
| title_eng |
Efecto del tipo de anticoagulante utilizado en la toma de muestras, sobre la determinación del perfil de acilcarnitinas en sangre y plasma. |
| description |
Antecedentes: la toma de muestras de sangre es un factor importante que puede influir los resultados finales de algunas pruebas de laboratorio. Las determinaciones de acilcarnitinas en sangre son utilizadas corrientemente para el diagnóstico y seguimiento de pacientes que sufren enfermedades metabólicas hereditarias. Objetivo: el presente estudio analizó la posible influencia del tipo de anticoagulante utilizado en la toma de la muestra de sangre, sobre el perfil de acilcarnitinas obtenido. Metodología: muestras de sangre y plasma fueron obtenidas utilizando heparina, potasio-EDTA, fluoruro de sodio-oxalato de potasio, y citrato de sodio y sin anticoagulante. Resultados: no fue encontrada diferencia estadísticamente significativa entre los anticoagulantes utilizados. Conclusión: el tipo de anticoagulante no tiene efecto sobre la determinación de acilcarnitinas en sangre o plasma. Para este tipo de análisis, puede ser utilizado cualquier anticoagulante de los antes mencionados. Abreviaturas: IEE-MS/MS, inyección de electrospray espectrometría de masas en tándem; EIM, errores innatos del metabolismo.
|
| description_eng |
Background: the collection process for blood sampling is a very important factor that can influence the final results of some laboratory tests. The acylcarnitine determinations in blood are usually used for diagnosis and follow up of patients suffering certain inherited metabolic illnesses. Objective: this study analyzed the possible influence of the type of anticoagulant used in blood sampling on the acylcarnitine profile. Methodology: blood and plasma samples were collected using anticoagulants such as: lithium heparin, potassium EDTA, sodium fluoride-potassium oxalate, and sodium citrate. Samples were also collected without the use of anticoagulants. Acylcarnitine levels were assessed for each sample via tandem mass spectrometry. Results: no significant differences were found among the anticoagulant used. Conclusion: the type of anticoagulant has no effect on the acylcarnitine determination on blood or plasma. For this type of analysis any of the above mentioned anticoagulants can be used. Abbreviations: ESI-MS/MS, electrospray injection tandem mass spectrometry; IEM, innate errors of metabolism.
|
| author |
Osorio, José Henry |
| author_facet |
Osorio, José Henry |
| topicspa_str_mv |
espectrometría de masas en tándem acilcarnitinas carnitina |
| topic |
espectrometría de masas en tándem acilcarnitinas carnitina carnitine acylcarnitines tandem mass spectrometry |
| topic_facet |
espectrometría de masas en tándem acilcarnitinas carnitina carnitine acylcarnitines tandem mass spectrometry |
| citationvolume |
9 |
| citationissue |
2 |
| citationedition |
Núm. 2 , Año 2010 : Julio - Diciembre |
| publisher |
Universidad de Caldas |
| ispartofjournal |
Biosalud |
| source |
https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498 |
| language |
Inglés |
| format |
Article |
| rights |
info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 Revista Biosalud - 2010 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| references_eng |
Osorio JH, Pourfarzam M. Plasma free and total carnitine measured in children by tandem mass spectrometry. Braz J Med Biol Res 2002;35(11):1265-71. Lepage N, Aucoin S. Measurement of plasma/serum acylcarnitines using tandem mass spectrometry. Methods Mol Biol 2010;603:9-25. Kodo N, Millington DS, Norwood DL, Roe CR. Quantitative assay of free and total carnitine using tandem mass spectrometry. Clin Chim Acta 1990 Jan 31;186(3):383-90. Osorio JH, Pourfarzam M. Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry. Acta Bioquim Clin Latinoam 2010;44(2):15-20. Holub M, Tuschl K, Ratschmann R, Strnadová KA , Mühl A , Heinze G, et al. Influence of hematocrit and localisation of punch in dried blood spots on levels of amino acids and acylcarnitines measured by tandem mass spectrometry. Clin Chim Acta 2006; 373:27–31. Sewell AC. Methods of carnitine analysis. In: Seim H, Löster H, editors. Carnitine-Pathobiochemical Basics and Clinical Applications. Bochum: Ponte Press 1996: 73-84. Osorio JH, Pourfarzam M. Determination of normal acylcarnitine levels in a healthy pediatric population as a diagnostic tool in inherited errors of mitochondrial fatty acid beta-oxidation. An Pediatr (Barc) 2007;67(6):548-52. Schoderbeck M, Auer B, Legenstein E, Genger H, et al. Pregnancy-related changes of carnitine and acylcarnitine concentrations of plasma and erythrocytes. J Perinat Med 1995;23:477-85. Mancinelli A, Iannoni E, Calvani M. Effect of temperature on the stability of long-chain acylcarnitines in human blood prior to plasma separation. Clin Chim Acta 2007; 375:169-170. Nagaraja D, Mamatha SN, De T, Christopher R. Screening for inborn errors of metabolism using automated electrospray tandem mass spectrometry: study in high-risk Indian population. Clin Biochem 2010;43(6):581-8. Stevens RD, Hillman SL, Worthy S, Sanders D, Millington DS. Assay for free and total carnitine in human plasma using tandem mass spectrometry. Clin Chem 2000;46(5):727-9. |
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https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5498 |
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